Extended Data Fig. 2: The effect of blocking nucleocytoplasmic transport on Ubc9Ts clearance.
(a) Quantitation of the percentage of cells containing nuclear or cytoplasmic inclusions in WT yeast expressing Ubc9Ts-EGFP after 120 minutes at 37 °C with and without treatment with 100μM MG132. A minimum of 500 cells per condition from 3 biologically independent experiments were counted and two-tailed Student’s t-tests were performed comparing the WT yeast without MG132 treatment to WT yeast with MG132 treatment using Prism software. P values were adjusted using two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli with a Q of 5%. Adjusted P value for nuclear no MG132 vs. +MG132 is 0.0035 and cytoplasmic no MG132 vs. +MG132 is 0.0011. Data are shown as mean values ± S.E.M. (b) Representative Structured Illumination super-resolution microscopy images taken of cells expressing EGFP-VHL after 2 hr at 37 °C with DMSO (left) or with 100μM MG132 (right) treatment. VHL is shown in green, nuclear pores in gold, and Hoechst counterstain in blue. Scale bars are 1μm. Numerical source data are available in source data.
