Extended Data Fig. 6: ESCRT involvement in the clearance of misfolded proteins. | Nature Cell Biology

Extended Data Fig. 6: ESCRT involvement in the clearance of misfolded proteins.

From: Nuclear and cytoplasmic spatial protein quality control is coordinated by nuclear–vacuolar junctions and perinuclear ESCRT

Extended Data Fig. 6

(a) Representative confocal images of WT yeast co-expressing Chm7-EGFP and either NLS-EGFP-LuciTs (left) or NES-DsRed-LuciTs (right) after 120 minutes at 37 °C and treated with 100μM MG132. Chm7 is shown in teal and remains diffuse throughout the cell, NLS-EGFP-LuciTs in green, NES-DsRed-LuciTs in purple, nuclear pores in gold and Hoechst counterstain in blue. Scale bar is 1μm. (b) Representative confocal images of WT and vps23Δ, vps34Δ, and vps15Δ yeast co-expressing NLS-EGFP-LuciTs and NES-DsRed-LuciTs after 2 hr at 37 °C and treated with 100μM MG132. NLS-EGFP-LuciTs is shown in green, NES-DsRed-LuciTs in purple, nuclear pores in gold, and Hoechst counterstain in blue. Insets show the budding INQ encapsulated by nuclear pores. Scale bars are 1μm. Same data as shown in Fig. 6c, but with the green channel separated to clearly detail the colocalization with the cytoplasmic protein.

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