Extended Data Fig. 1: Spatial sequestration occurs during different types of stress with different client proteins. | Nature Cell Biology

Extended Data Fig. 1: Spatial sequestration occurs during different types of stress with different client proteins.

From: Nuclear and cytoplasmic spatial protein quality control is coordinated by nuclear–vacuolar junctions and perinuclear ESCRT

Extended Data Fig. 1

(a) Western blot analyses of Gal Shut-off assays showing the clearance of NLS-LuciTs (top) and NES-LuciTs (bottom) with and without proteasome impairment by 50μM Bortezomib. Blot is representative of 3 biologically independent experiments. (b-c) Representative Structured Illumination super-resolution microscopy images taken of cells expressing NLS-VHL (b) or NES-VHL (c) after 120 minutes at 37 °C and treated with 100μM MG132. NLS-LuciTs is shown in green, NES-LuciTs in purple, nuclear pores in gold and Hoechst counterstain in blue. Scale bars are 1μm. (d) Drop test of W303 yeast expressing model proteins without heat shock at 30C (left), with heat shock at 37 °C (middle), and without expression of the plasmids (right). Unprocessed blots are available in source data.

Source data

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