Extended Data Fig. 4: Single-cell Multiomic ATAC and gene expression characterization of primary huma islets.
a, Brightfield images of primary islets used for multiomic sequencing. Individual image representative of 1 sample. b, Brief primary islet donor information. c, Heatmap showing ATAC promoter accessibility of markers associated with each cell type (30202 cells from 4 independent biological samples; integration of all samples). d, Violin plots for INS, GCG, SST, and PPY gene expression in primary islet cell types from different donors. e, Heatmaps showing the top 20 enriched motifs in primary β, α, δ, and PP cells. f, Re-clustering analysis of primary β cells highlighting heterogeneity by variations in mature and polyhormonal gene expressions. g, Dotplots showing marker genes and unique genes associated with subpopulations identified from the re-clustering of primary β cells. h, Dotplots showing unique motif chromatin accessibility in in subpopulations primary β cells. i, Chromatin accessibility of transcription factor binding sites of MAF, RFX, FOS/JUN, and TEAD motif family in primary β cell subpopulations. PP, Pancreatic progenitors.
