Extended Data Fig. 5: Multiomic sequencing comparison of SC-islets and primary human islets.
a, Integrative UMAP showing cells from SC-islets and primary islets (47566 cells from 5 independent biological samples; 3 SC-islets, 2 representative primary islets- donor #1 and #2). b, Integrative UMAP plotted by SC-islet or primary islet condition. Pie charts show composition information of cell types identified. c, Validation analysis showing integrative UMAP of cells from SC-islets and other primary islet datasets (41688 cells from 5 independent biological samples; 3 SC-islets, 2 representative primary islets- donor #3 and #4). Pie charts show composition information of cell types identified. d, Validation analysis showing heatmap highlighting and comparing identity (β, adult β, α, δ, PP and EC) associated gene expression and ATAC promoter accessibility of endocrine cells in SC-islets and primary islets (Human islet donors #3 and #4). e, Validation analysis showing heatmap highlighting and comparing off-target identity (Exocrine, hepatic, esophagus, stomach, intestinal, pancreatic progenitor) associated gene expression and ATAC promoter accessibility in SC-islet cells and primary islet cells (Human islet donors #3 and #4). f, Differential gene expression (top) and motif chromatin accessibility analysis (right) for α-cells (left) and δ-cells (right). Statistical significance was assessed by Wilcoxon rank sum test for RNA expression and logistic regression for motif chromatin accessibility. g, Bar graphs showing fold change comparing SC and primary α and δ cell populations, showing gene expression and motif chromatin accessibility of identified transcription factors associated with the respective cell types. EC, enterochromaffin cells.
