Extended Data Fig. 7: Single-cell Multiomic ATAC and gene expression characterization of transplanted SC-islets.
a, Schematics of SC-islet transplantation and retrieval of graft after 6 months in vivo. b, UMAP and identification of transplanted SC-islet cells using gene expression and chromatin information (7162 cells from 3 independent biological samples; integration of all samples). c and d, Heatmap showing gene expression (c) and ATAC promoter accessibility (d) of markers associated with each cell type. e, Pearson correlation analysis using top 2000 most variable ATAC promoter accessibility of key endocrine populations from SC-islets, transplanted SC-islets, and primary human islets. This analysis highlights the distinctiveness of chromatin identity acquired in cell types from SC-islets after transplantation. f, Heatmap showing the top 200 variable motifs within endocrine cell populations and highlighting motif markers for each in vivo cell type. g, Heatmaps highlighting gene expression and ATAC motif accessibility of top 10 active transcription factors co-enriched with both features in transplanted SC-β, SC-α, SC-δ, and SC-EC cells.
