Extended Data Fig. 1: Single-cell Multiomic ATAC and gene expression characterization of SC-islets.
a, Schematic diagram outlining the differentiation protocol for generating SC-islets. b, Brightfield images of differentiated SC-islets from the three batches used for multiomic sequencing. Individual image representative of 1 sample. c, Heatmap showing ATAC promoter accessibility of markers associated with each cell type (29526 cells from 3 independent differentiations; integration of all samples). d, Violin plots for INS, GCG, SST, and TPH1 gene expression in SC-islet cell types from different datasets of different differentiation batches. e, Heatmaps showing the top 20 enriched motifs in SC-EC1, SC-β, SC-α, SC-δ, and SC-EC2 cells. f, Pearson correlation analysis using the top 2000 variable genes comparing correlation of SC-β, SC-α, and SC-EC in SC-islets from this study and other literatures using other differentiation protocols. SC-β, and SC-α have similar gene expression profiles across multiple datasets. g, Dot plots showing the upregulation, by gene expression, of identified active transcription factors in SC-β, SC-α, and SC-EC cells of datasets from other studies. h, Feature plot showing gene expression of progenitor transcription factors and chromatin accessibility of their binding motifs. EC, enterochromaffin cells.
