Extended Data Fig. 7: The metabolic response of LONP1 AKO mice to dietary succinate supplementation.
From: Proteolytic rewiring of mitochondria by LONP1 directs cell identity switching of adipocytes
(a) iWAT succinate content in mice subjected to dietary succinate supplementation. 4-week-old WT and LONP1 AKO mice fed with or without 1.5% sodium succinate drinking for 2 weeks. n = 5 mice per group. (b) Quantification of the UCP1/Tubulin signal ratios from iWAT of vehicle or CL316,243 treated WT and LONP1 AKO mice fed with or without sodium succinate in Fig. 6f. n = 5 mice per group. (c) Body composition measured to determine fat mass and lean tissue mass of 4-week-old WT and LONP1 AKO mice fed with or without 2 weeks sodium succinate water. nWT Con = 9; nWT Succinate = 5; n AKO Con = 5; nAKO Succinate = 5 mice. (d) Growth curve of WT and LONP1 AKO mice. nWT = 14; n AKO = 12 mice. (e) Fat mass and lean tissue mass of indicated mice at 4 months of age, nWT = 9; n AKO = 6 mice. (f) Bodyweight of 4-month-old WT and LONP1 AKO mice fed with or without sodium succinate water for 10 weeks. nWT Con = 7; nWT Succinate = 6; n AKO Con = 7; nAKO Succinate = 6 mice. (g-k) Metabolic evaluation of WT and LONP1 AKO mice using a comprehensive laboratory animal monitoring system (CLAMS). nWT Con = 11; nWT Succinate = 6; n AKO Con = 8; nAKO Succinate = 6 independent mice. (g) Food consumption. (h) Locomotor activity. (i) Energy expenditure. (j) Oxygen consumption. (k) Carbon dioxide production. (l) Left: Glucose tolerance test (GTT). Right: Area under the curve for GTT is shown. (m) Left: Insulin tolerance test (ITT). Right: Area under the curve for ITT is shown. (l, m) nWT Con = 6; nWT Succinate = 7; n AKO Con = 7; nAKO Succinate = 7 mice. All data are shown as the mean ± SEM. P value was calculated by unpaired two-sided Student’s t-test (e), or two-way ANOVA (a-d, f-m). Source numerical data are available in source data.
