Extended Data Fig. 3: LIPTER deficiency impairs survival of long-term cultured hiPSC-CMs.
From: Lipid droplet-associated lncRNA LIPTER preserves cardiac lipid metabolism
a, LIPTER was knocked out in hiPSCs using CRISPR /Cas-9. Dual gRNAs were designed to completely ablate LIPTER. Genotyping was carried out in individual hiPSC clones using PCR primer sets for detecting long deletions. b, Ratios of beating EBs during CM differentiation from WT and two LIPTERKO hiPSC lines. Dots represent mean values ± s.e.m. Unpaired two-tailed t-test is used for comparison. No significance detected. (n = 3 independent experiments). c, Representative FACS results of cTnT+ cells ratios at day 20 differentiation of WT and LIPTERKO hiPSC lines. d, Quantification of cTnT+ cell ratios at day 20 of differentiation. Bars represent mean values, (n = 2 independent experiments). e, RT-qPCR detecting expressions of CMs markers CTNT, MYH6 and MYH7 in WT and LIPTERKO hiPSC-EBs at day 20 of differentiation. Bars represent mean values, (n = 2 independent experiments). f, Representative FACS results of cTnT+ CM ratios in WT and LIPTERKO hiPSC-EBs at day 40 of differentiation. g, Nile Red and cTnT co-staining in WT, LIPTERKO and LIPTERKO/OE hiPSC-derived EB sections. h, Quantification of Nile Red positive areas in cTnT+ CM areas. Bars represent mean values ± s.e.m. Unpaired two-tailed t-test is used for comparison. ***p < 0.001. (n = 4 independent experiments). i, RT-qPCR detection of LIPTER overexpression levels in LIPTERKO/OE hiPSC-CMs treated with various concentrations of doxycycline for 4 days. Bars represent mean values ± s.e.m. Unpaired two-tailed t-test is used for comparison. ****p < 0.0001, **p < 0.01, n.s (no significance). (n = 3 independent experiments). Source numerical data are available in source data.
