Extended Data Fig. 5: NKX2.5 deficiency reduces LIPTER expression and phenocopies LIPTERKO hiPSC-CMs.
From: Lipid droplet-associated lncRNA LIPTER preserves cardiac lipid metabolism
a, RT-qPCR analysis of cTnT, LIPTER, MYH10 and NKX2.5 expressions in WT hiPSC-CMs after infection with AAV9 virus carrying scramble control shRNA or two NKX2.5-shRNAs. (n = 5 independent experiments). b, Representative images of Oil Red O lipid staining for LD accumulation, and NKX2.5, cTnT, DAPI immunostaining in WT, LIPTER KO and LIPTER KO/OE hiPSC-CMs treated with control shRNA or NKX2.5-shRNA under low (5.5 mM) or high glucose (22 mM) conditions. The experiment was carried out 4 times with similar outcomes. c, Quantification of the ratios of Oil Red O positive areas in cTnT+ CM areas in b. (n = 4 independent experiments). d, Representative immunofluorescent images of TUNEL, NKX2.5, cTnT and DAPI staining in WT, LIPTER KO and LIPTER KO/OE hiPSC-CMs treated with control or NKX2.5 shRNA under high glucose conditions (22 mM). The experiment was carried out 4 times with similar outcomes. e, Quantification of the ratios of TUNEL+ CMs in cTnT+ CMs of (d). (n = 4 independent experiments). In a,c,e, bars represent mean values ± s.e.m. Unpaired two-tailed t-test is used for comparison. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05. Source numerical data are available in source data.
