Extended Data Fig. 7: Selective binding of LIPTER with PA/PI4P and MYH10.
From: Lipid droplet-associated lncRNA LIPTER preserves cardiac lipid metabolism
a, RT-qPCR analysis of LIPTER, U6 (nuclear RNA) and GAPDH (cytosolic RNA) expression levels in fractionated nuclei and cytoplasm of hiPSC-CMs. Bars represent mean values ± s.e.m. (n = 3 independent experiments). b, Representative images showing no detectable interactions of giant lipid vesicles generated by TopFluor-PI with Alexa594-labeled LIPTER or Antisense-LIPTER (AS-LIPTER). The experiment was carried out 3 times with similar outcomes. c, A specific protein band only pulled down by LIPTER-MS2 in HEK293T cells and visualized in SDS PAGE gel by silver staining. MS2 binding protein was FLAG-tagged. The experiment was carried out 2 times with similar outcomes. d, Western blotting confirming MYH10 pulldown by LIPTER in HEK293T cells. The experiment was carried out 2 times with similar outcomes. e, 3D confocal immunofluorescent images showing colocalization of LIPTERYFP-MS2, MYH10, and LD in WT hiPSC-CMs (also see Supplementary Video 1). The lower right graph indicates the view of merged images. The experiment was carried out 3 times with similar outcomes. f, Immunofluorescent images showing MYH10-ACTIN cytoskeleton in WT hiPSC-CMs. The experiment was carried out 3 times with similar outcomes. Source numerical data and unprocessed blots are available in source data.
