Extended Data Fig. 10: CM-targeted LIPTER transgene exhibits cardiac protective effects in Leprdb/db mice.
From: Lipid droplet-associated lncRNA LIPTER preserves cardiac lipid metabolism
a, Heart images of WT and db/db mice 6 weeks after injection of AAV9-cTnT-GFP, AAV9-cTnT-AS-LIPTER or AAV9-cTnT-LIPTER, with fluorescent images showing robust GFP expression post AAV9-cTnT-GFP injection. The experiment was carried out 4 times with similar outcomes. b, Representative images showing GFP expression in CMs of db/db mouse heart 6 weeks after AAV9-cTnT-GFP injection. The experiment was carried out 4 times with similar outcomes. c, RT-qPCR examining GFP expression levels in WT and db/db mouse hearts 6 weeks after AAV9 injection. (n = 5 mice per group, except n = 4 mice in the GFP group). d, Representative images of WGA staining in WT or db/db mouse hearts 6 weeks after AAV9 injection. The experiment was carried out 5 times with similar outcomes. e, Blood glucose levels of WT and db/db mice 6 weeks after AAV9 injection. WT mice without AAV9 injection also serve as control. (n = 5 mice per group, except n = 4 mice in the GFP group). f, EMSA results showing no direct interaction between LIPTER and TFG protein. The experiment was carried out 2 times with similar outcomes. In c and e, bars represent mean values ± s.e.m. Unpaired two-tailed t-test is used for comparison. ***p < 0.001, **p < 0.01. Source numerical data are available in source data.
