Extended Data Fig. 6: P2 of STING is required to associate with HK2 and suppress HK2 activity.
From: STING is a cell-intrinsic metabolic checkpoint restricting aerobic glycolysis by targeting HK2
a, The Pearson’s correlation coefficients between HK2 and mitochondria. b, Sting1−/− MLF cells stably expressing EGFP-mSTING were labelled with MitoTracker and stimulated with DMXAA (25 μg/ml) for 30 min. After immunostaining with HK2 antibody, cells were imaged by STED super-resolution microscopy. Scale bars, 5 μm. c, Sting1−/− MLF cells stably expressing EGFP-mSTING were stimulated with DMXAA (25 μg/ml) for 30 min. After immunostaining with HK2 and GM130 (a Golgi marker) antibodies, cells were imaged by STED super-resolution microscopy. Scale bars, 5 μm. d and e, FLAG-HK2 and HA-tagged STING (1–160) or the indicated mutants were co-expressed in HEK293T cells. WCLs were immunoprecipitated with anti-FLAG agarose and analysed by immunoblotting. f, Reporter assays for IFNB1 promoter activity in HEK293T cells transfected with HA-tagged STING containing the indicated mutations and FLAG-cGAS (Left). WCLs were analysed by immunoblotting (Right). Data are presented as means ± s.d. of n = 8 (a) or n = 3 (f) independent biological replicates. Statistical analyses were performed using a two-tailed unpaired Student’s t-test. Data are representative of three independent experiments. Source numerical data and unprocessed blots are provided in Source Data.
