Extended Data Fig. 9: STING restricts aerobic glycolysis and promotes antitumour immunity in established tumours.
From: STING is a cell-intrinsic metabolic checkpoint restricting aerobic glycolysis by targeting HK2
a, the weight of the tumours at 14 days post-transplantation as described in Fig. 6b was shown (n = 6 in each group). b, Flow cytometry analysis of tumour-infiltrating lymphocytes in MC38 tumours as described in Fig. 6b (n = 6). c, Flow cytometry analysis of tumour-infiltrating CD4+ T cells and the severe exhausted CD4+ cells in MC38 tumours as described in Fig. 6b (n = 6). d and e, Flow cytometry analysis of CD69+ (d) or CD44+ (e) cells in tumour-infiltrating CD8+ cells in MC38 tumours as described in Fig. 6b (n = 6). f, the weight of the tumours at 16 days post-transplantation as described in Fig. 6g was shown (n = 5). g, Flow cytometry analysis of lymphocytes in MC38 tumours as described in Fig. 6g (n = 5). h and i, Flow cytometry analysis of tumour-infiltrating CD4+ T cells in lymphocytes (h) and the severely exhausted CD4+ T cells (i) in MC38 tumours as described in Fig. 6g (n = 5). j, Measurement of lactate levels in serum, lung, spleen, brain, heart and skeletal muscle of Sting1+/+ and Sting1−/− mice (n = 10 in Sting1+/+ group and n = 7 in Sting1−/− group). k and l, representative figures showing gating strategies for all flow cytometry analysis in this study. Data are presented as means ± s.d., and statistical analyses were performed using a two-tailed unpaired Student’s t-test. Data are representative of three independent experiments. Source numerical data and unprocessed blots are provided in Source Data.
