Extended Data Fig. 7: Blockage of FASN affects mTORC1 activity independently of AMPK and the GATOR1 complex.
From: Malonyl-CoA is a conserved endogenous ATP-competitive mTORC1 inhibitor
a–c, WT or AMPKα1/2 KO HEK293T cells were treated with 25 μM Fasnall (30, 60 min) or 50 μM Cerulenin (2, 4 h). b,c, Quantification of mTORC1 activity (p-S6KT389/S6K) for Fasnall (b), normalized to the respective DMSO-treated control, and cerulenin (c) treatment; n = 3 independent experiments. d,e, As in a, but for FASN knockdown. e, Quantification of mTORC1 activity (p-S6KT389/S6K), normalized to each siCtrl sample; n = 3 independent experiments. f–h, As in a–c, but in DEPDC5/RagA/RagC-triple-knockout cells. g,h, Quantification of mTORC1 activity (p-S6KT389/S6K) for Fasnall (g), normalized to the respective DMSO-treated control, and cerulenin (h) treatment; n = 3 independent experiments. i,j, As in f–h, but for FASN knockdown. j, Quantification of mTORC1 activity (p-S6KT389/S6K), normalized to the respective siCtrl sample; n = 4 independent experiments. Data in graphs are shown as the mean ± s.e.m. *P < 0.05, **P < 0.005, ***P < 0.0005. Source numerical data and unprocessed blots are provided.
