Extended Data Fig. 6: Genome-wide macroH2A2 occupancy in cultured WT CAFs.
a) Correlation of genome-wide enrichment of macroH2A between ChIP-seq and CUT&RUN methodologies. ChIP-seq and associated inputs were previously generated in dermal fibroblasts62, CUT&RUN was performed in serum-starved unstimulated and serum-stimulated WT CAFs, with associated IgG control. Pearson’s correlation coefficients shown, conditions are clustered based on Euclidean distance. b) Metagene profile of macroH2A CUT&RUN signal in cultured WT CAFs before and after serum stimulation across genes binned into quartiles according to their expression levels. Genes not detected are either not expressed or not mappable. n = 3000 randomly selected genes within each group. c) Metagene profile of macroH2A CUT&RUN signal in cultured WT CAFs before and after serum stimulation at different classes of MCDs and genomic regions where MCDs are absent. d) Size distributions of regions in (c). Box plot center line represents the median, box plot limits – 25th to 75th percentiles, whiskers extend from the box limit to the most extreme value no further than 1.5 * inter-quartile range from the box limit. c-d, nsuper = 1560, nstandard = 5440, nlow = 1556, nabsent = 23615 regions. e) Proportion of the genome comprised within different classes of MCDs.
