Extended Data Fig. 6: Borealin K143 methylation regulates AurkB activity at kinetochores.
From: Non-canonical MLL1 activity regulates centromeric phase separation and genome stability
(a-b) Immunofluorescence for CENPApS7 or H3pS10 in HeLa cells treated with control or MLL1 siRNAs (a) or in Borealin WT or K143R cells (b). Representative images from two independent experiments were presented. Scale bar, 10 μm. (c-f) Immunofluorescence for Hec1 pS44 (c), Dsn1 pS109 (d), Knl1 pS60 (e), and AurkB pT232 (f) as well as HA-Borealin in synchronized prometaphase WT or K143R cells as indicated on left. DNA was stained with Hoechst. Representative images from three independent experiments were presented. Scale bar, 10 μm. (g-j) Quantification of signals of respective phosphorylated proteins in (c-f) after normalizing against HA-Borealin. The graphs show the mean and S.D. A total of 15 cells were quantified per condition. Data are representative of two independent experiments. The p value was calculated using two-tailed unpaired Student’s t-test.
