Fig. 1: DmTMEM63 localizes to lysosomes.
From: Drosophila TMEM63 and mouse TMEM63A are lysosomal mechanosensory ion channels
a, Representative images showing the expression patterns of the DmTMEM63 proteins and organellar markers in S2 cells. b, Quantification of the co-localization between DmTMEM63 and lysosome (n = 11 cells), mitochondrion (n = 10 cells) or peroxisome (n = 10 cells). Data shown are mean ± s.e.m. c, Schematic representation of the genomic locus of Tmem63 in WT and DmTmem63FP (FP, fluorescent protein) knock-in flies. GFP or mCherry was inserted to follow the last amino acid of the endogenous DmTMEM63 protein. The green triangle and blue bar indicate the start codon and stop codon, respectively. d, Immunoblots of head extracts in WT, DmTmem63GFP and DmTmem63mCherry flies showing the presence of full-length fusion proteins. e, Co-localization of DmTMEM63 proteins with lysosomal markers in VNC motor neurons and fat bodies of the wandering-stage DmTmem63mCherry fly larvae. Lysosomes were labelled by GMR51B08-Gal4-driven Spin–GFP or Cg-Gal4-driven GFP–LAMP1. f, Representative images showing the expression of DmTMEM63 proteins and LysoTracker-Red signals in the fat body of the DmTmem63GFP wandering-stage larvae. LysoTracker-stained acidic organelles. For d–f, the results are representative of three independent experiments. For e and f, dashed lines indicate a single cell within the tissues. Scale bars, 5 µm (a,e and f). Numerical data and unprocessed blots are available as Source data.
