Extended Data Fig. 1: ATGL-driven lipolysis is increased in the liver and adipose depots from fasted and glucose-deprived mice.
From: Glucose controls lipolysis through Golgi PtdIns4P-mediated regulation of ATGL
(a) Immunoblots of ATGL, HSLSer660ph, HSL, MGL, PLIN1, CGI-58 and HSP90 in the liver and adipose depots from fed and fasted mice. (b) Plasma glucose levels in the wild-type mice 1 h, 6 h and 12 h after 2-DG (2 g/kg BW) administration (1 h and 6 h, n = 6 mice for saline and 8 mice for 2-DG group; 12 h, n = 6 mice for saline and 7 mice for 2-DG group). (c–e) Levels of glucose and glucose derivatives (glucose-6-p and GA3P) in the livers of wild-type mice collected 1 h and 6 h after 2-DG (2 g/kg BW) administration (n = 6 mice for saline and 8 mice for 2-DG group). (f) Plasma insulin levels in the wild-type mice 1 h, 6 h and 12 h after 2-DG (2 g/kg BW) administration (1 h and 6 h, n = 6 mice for saline and 8 mice for 2-DG group; 12 h, n = 6 mice for saline and 7 mice for 2-DG group). (g) Immunoblots of ATGL, HSLSer660ph, HSL and HSP90 in the liver and adipose depots collected 1 h, 6 h and 12 h after 2-DG (2 g/kg BW) administration. (h) Quantification of HSLSer660ph levels in the adipose depots collected 1h, 6 h and 12 h after 2-DG (2 g/kg BW) administration (1 h and 6 h, n = 6 mice for saline and 8 mice for 2-DG group; 12 h, n = 12 mice for saline and 15 mice for 2-DG group). Results are shown as mean ± s.e.m. and analyzed using two-tailed unpaired t-test (b (12 h), c (1 h), d (6 h), e, f and h (1 h and 12 h in iBAT, iWAT and gWAT)) and two-tailed Mann-Whitney test (b (1 h and 6 h), c (6 h), d (1 h) and h (6 h in iBAT)). Source numerical data and unprocessed blots are available in source data.
