Extended Data Fig. 8: Generation GFP-KO cell lines and microscopic analyses of chimeric organoids.
From: Cerebral organoids display dynamic clonal growth and tunable tissue replenishment
a-f, Generation of GFP-KO-ASPM (a, b), GFP-KO-TP53 (c, d) and GFP-KO-PAX6 (e, f) cell lines. Sanger sequencing tracks on the left and TIDE analysis of genomic DNA (a, c, e). Protein expression analysis in (b, d, f). Immunoblotting was preferred for protein expression, however, ASPM antibody did not recognize blotted protein. g, Immunofluorescent images of chimeric organoids containing RFP-WT and indicated GFP-KO cells. RFP and GFP merged images on the left with individual signals shown as indicated. SOX2 and NeuN signal marks neural progenitors and neurons respectively.
