Extended Data Fig. 1: Details on whole organoid lineage tracing.
From: Cerebral organoids display dynamic clonal growth and tunable tissue replenishment
a, 2D barcoded lineage tracing data. Normalized reads per lineage (RPM) plotted for the indicated time points for each barcode. Individual Barcode Libraries 1 and 2 are indicated. (n = individual lineages of individual biological replicates from 1 experiment at each time point are shown). b, 3D lineage tracing data. Normalized reads per lineage (RPM) plotted for the indicated time points for each barcode. Individual Barcode Libraries 1 and 2 are indicated. (n = individual lineages of individual organoids as biological replicates from 1 differentiation experiment at each time point are shown). c, Contribution to organoids of ranked 5 percentiles of lineages over time in 3D organoids, normalized to whole organoids. (n = individual lineages from 3 organoids as biological replicates from 1 differentiation experiment at each time point). d, Cumulative contributions of lineages to whole 3D organoids over all time points. (n = individual lineages from 3 organoids as biological replicates from 1 differentiation experiment at each time point).
