Extended Data Fig. 3: MeRIP-Seq shows different m6A epi-transcriptome profiling of Poly(A) + RNA in se and mta vs Col-0.
From: SERRATE drives phase separation behaviours to regulate m6A modification and miRNA biogenesis
a, HPLC-MS quantification of m6A/A levels of commercial m6A (+) and m6A (–) spike-ins, GLuc (m6A/A, ~20%) and CLuc, respectively. Data are mean ± s.d. of three independent experiments. b, Schematic approach for MeRIP-Seq. Briefly, the purified poly(A) + RNA was mixed with internal controls containing m6A modified (red) and unmodified (yellow) spike-ins, and then fragmented. The resulting fragments were immunoprecipitated using a specific anti-m6A antibody. Parallel IPs using an anti-GFP antibody were performed as negative controls. The IP-ed RNAs were then processed for library construction and high-throughput sequencing, enabling the identification and quantification of m6A modifications at a transcriptome-wide level. c, Autography images of input and m6A RNA enriched by indicated antibodies in (b). One tenth of the input and all of immunoprecipitated RNA were de-phosphorylated and then labelled with P32-ATP before resolvement in 8% urea gels. d, The motif sequences for m6A modifications in the context.
