Extended Data Fig. 8: Analysis of human colon organoids. | Nature Cell Biology

Extended Data Fig. 8: Analysis of human colon organoids.

From: Chromatin remodelling in damaged intestinal crypts orchestrates redundant TGFβ and Hippo signalling to drive regeneration

Extended Data Fig. 8

a) Schematic of experimental design (top) and representative bright-field images of human colon organoid (HCO) cultures (bottom). P, passage number; d, day. +/- denotes presence or absence of TGFβ/inhibitor. Scale bar, 500 µm. b) qRT-PCR analysis indicating changes in revSC marker expression (CLU, F3, ANXA1) and SMAD7. Expression normalized to GAPDH, ‘+A-83-01/-TGFβ1’ represents the control. The mean±range of N = 2 replicates, performed in triplicate, is plotted. Source numerical data available in source data.

Source data

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