Extended Data Fig. 9: Cohesin depletion does not alter rate of resection following DSB.
From: Cohesin complex oligomerization maintains end-tethering at DNA double-strand breaks
a, b, Spot characteristics of individual cells inrad52∆ and rad51∆ cells during a 12 hour period after DNA DSB induction. Yellow represents presence of red and green spots, red represents presence of red spot only, green represents presence of green spot only, grey represents the presence of no spots, and white represents cell death. Images were acquired every ten minutes at each position on a microfluidic plate. Data represent N = 61 (rad52∆), N = 54 (rad51∆) cells from n = 2 independent experiments c, Time taken for loss of both red and green spots after DSB induction in microfluidic experiments for SMC1-AID strains shown in Fig. 6e,f. Red line at median, quartiles represented by dashed line. Data represent N = 77 (+Smc1) and N = 79 (-Smc1) cells from n = 2 independent experiments. d, e, Spot characteristics of individual cells in CDC45-AID, CDC45-AID SMC1-AID cells during a 15.5 hour period after DNA DSB induction. DSB was induced in the microfluidic plate, and images were acquired every ten minutes at each position on a microfluidic plate. Data represent N = 45 (-Cdc45) and N = 47 (-Cdc45 -Smc1) cells from n = 3 (-Cdc45) and n = 2 (-Cdc45 -Smc1) independent experiments. f, Time taken for loss of both red and green spots after DSB induction in microfluidic experiments for auxin exposed CDC45-AID and CDC45-AID SMC1-AID strains shown in panels d-e. Red line at median, quartiles represented by dashed line. P values were calculated using a two-tailed unpaired Mann Whitney test. (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001, not significant (ns) P > 0.05). Numerical P values are indicated in Supplementary Information Table 4. (c) + Smc1 vs -Smc1 P = 0.5292. (d) -Cdc45 vs -Cdc45 -Smc1 P = 0.0731. Source numerical data are available in Source data.
