Extended Data Fig. 5: Single-cell analysis of iPSC-derived NPCs reveals diverging identity.
From: The enhancer module of Integrator controls cell identity and early neural fate commitment
(a-b) Normalized expression of INTS10 (a) and SOX2 (b) in each cell on UMAP plots shows uniformly reduced INTS10 expression in HET2 NPCs, whereas changes in SOX2 expression between WT and HET2 NPCs are not significant. (c) SingleR annotations from the Human Primary Cell Atlas (which does not contain a NPC cell type) were used to build a heatmap of the 4 annotated clusters in Fig. 5b. The color scale reflects the percentage contained in each cluster for every cell type. Cluster 1 is primarily composed by neuron-like cells, whereas cluster 2 contains a variety of hematopoietic prognitors (likely mis-annotation of mesenchymal cells). Cluster 3 contains a higher proportion of iPS cells and cluster 4 is primarily composed by neuroepithelial cells. (d) Violin plots showing normalized expression of select marker genes in WT and HET2 cells.
