Extended Data Fig. 5: scRNA-seq analysis of Sox1-GFP+ pNSCs.

a, Dot plot showing the top 4 markers for each cluster in all Sox1-GFP+ cells. b, Heatmap of the expression of the top 50 marker genes in different Sox1+ cell populations. Each column represents a cell and each row represents a gene. c, UMAP showing the expression of representative markers for each population in Sox1-GFP+ cells. d, UMAP showing the expression of NSC markers in brain and tail Sox1-GFP+ cells. e,f, Violin plots showing the expression of NSC and Schwann cell/NC-derived cell marker genes in different populations of brain, tail Sox1-GFP+ cells (e) and Sox2-GFP+ lung Schwann cells (f). n = 1 biological replicate for all the scRNA-seq samples.