Extended Data Fig. 7: Comparison of pNSCs with other tissue stem cells and the origin of ldNSCs and pNSCs. | Nature Cell Biology

Extended Data Fig. 7: Comparison of pNSCs with other tissue stem cells and the origin of ldNSCs and pNSCs.

From: Multipotent neural stem cells originating from neuroepithelium exist outside the mouse central nervous system

Extended Data Fig. 7

a,b, Pearson correlation (a) and hierarchical clustering (b) of tail pNSCs with brain NSCs, lung AT1 and AT2 stem cells, and hair follicle epithelium stem cells. n = 1 biological replicate. c,d, FACS strategy for postnatal lung Sox1-GFP+ (c) and Sox1-GFP- (d) cells, and representative morphologies of pNSC cluster and Sox1-GFP- cells after low-pH treatment and culture, as assessed by bright-field (BF) microscopy. e, NSC-like cluster forming efficiency from the postnatal tail and brain Sox1-GFP+ cells with or without low-pH treatment. The data represent means ± s.d. (n = 3 biological replicates). f, In Sox1-Cre-mTmG embryos, GFP expression was detected in the neuroepithelial cells at E8.5, as assessed by bright-field (BF) microscopy, GFP and Tomato signals. Data represent 3 biological replicates. g, Immunohistological analysis of E8.5 Sox1-Cre-mTmG embryos using antibody against GFP. Data represent 3 biological replicates. Scale bar, 50 μm. h, Lung pNSCs and tail pNSCs from postnatal Sox1-Cre-mTmG mouse could be stably maintained for more than 50 passages in monolayer, as assessed by bright-field (BF) microscopy, GFP and Tomato signals. i, Immunofluorescence microscopy images of lung pNSCs, tail pNSCs and brain NSCs from postnatal Sox1-Cre-mTmG mouse using antibodies against Sox1, Sox2, Olig2, Nestin, Sox10 and p75. j, Expression levels of NSC marker genes in different samples quantified by RT-qPCR, normalized to brain NSCs. The data represent means ± s.d. (n = 3 biological replicates). k, Lung pNSCs, tail pNSCs and brain NSCs from postnatal Sox1-Cre-mTmG mouse could differentiate into neurons (Tuj1+), astrocytes (GFAP+) and oligodendrocytes (MBP+) in vitro. l, The in vitro differentiation efficiencies of pNSCs and brain NSCs from Sox1-Cre-mTmG mouse into neurons, astrocytes, and oligodendrocytes were quantified and compared via immunostaining with Tuj1, GFAP, and MBP, respectively. Brain control NSCs were used as a positive control for determining the relative differentiation efficiency. The data represent means ± s.d. (n = 3 biological replicates).

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