Extended Data Fig. 6: The pericentriolar material (PCM) superscales upon differentiation.
From: Cell state-specific cytoplasmic density controls spindle architecture and scaling
a. Confocal micrographs (maximum projected, representative from ESCs n = 113 cells and DIF n = 100 cells pooled from 3 independent experiments) of fixed ESCs or DIF at metaphase. Top: Immunostained CDK5RAP2 signal (inverted grayscale), bottom: immunostained CDK5RAP2 (yellow), tubulin::GFP (grey) and chromatin (Hoechst, blue). Dotted lines indicate cell boundaries. Scale bar: 5 µm. b. As in a) but using CEP192 (representative from ESCs n = 56 cells and DIF n = 44 cells from 1 experiment). c. As in a) but using pericentrin (representative from ESCs n = 89 cells and DIF n = 73 cells pooled from 3 experiments). d. Scaling relationship between cell volume and centrosome volume based on CDK5RAP2. Data points represent single cells (ESCs n = 113 cells and DIF n = 100 cells pooled from 3 independent experiments), large circles denote medians per cell volume bin, the error bars show interquartile ranges. e. As in d) but based on CEP192 (ESCs n = 56 cells and DIF n = 44 cells pooled from 1 experiment). f. As in d) but based on pericentrin (ESCs n = 89 cells and DIF n = 73 cells pooled from 3 experiments).
