Extended Data Fig. 3: CD44-mediated iron endocytosis prevails in the mesenchymal state of cells.
From: CD44 regulates epigenetic plasticity by mediating iron endocytosis
a, Time course flow cytometry of CD44 and TfR1 at plasma membrane of MCF7 and HMLER CD44low cells treated with OSM or TGF-β as indicated. Data representative of n = 3 biologically independent experiments. b, Fluorescence microscopy of RhoNox-M-positive vesicles colocalizing with internalized Hyal-FITC or TF-647 in cells treated as indicated for 72 h. Scale bars, 10 μm. n = 3 biologically independent experiments for MCF7 cells and n = 1 for primary cells. Bars and error bars, mean values ± s.d. Unpaired t-tests, two-sided.
