Extended Data Fig. 3: An overview of the central nervous system (CNS) of the spider.
From: Single-cell transcriptomics reveals the brain evolution of web-building spiders
a, different z-section layer showed the different brain part by Synapsin (anti-SYNORF1, green) staining. b, Schematic diagrams showing the organization of spider brain in side view. grey line with different z value corresponding the z-section of confocal laser scanning. c, Photograph of an adult male Hylyphantes graminicola and the brains used for immunolabeling. d, Schematic diagrams showing the organization and major structure of the spider CNS in dorsal view. EL, eye laminae (red): CB, central body (blue); CL, corpus lamellosum (yellow); MB, mushroom body (green); PPN, pedipalpal neuropil; WLN1-4, walking leg neuromere 1-4; OPIN, opistosomal nerves. During dissection, staining, and slide preparation, protocerebrum (including EL, CB, CL, MB) and subesophageal mass (including WLN) are fragile and only with loose connection. So they are almost at same horizontal level and the WLN thus are visible at different z-section.
