Extended Data Fig. 3: NMF analysis.
From: Cell type and cell signalling innovations underlying mammalian pregnancy
a-f. Optimal numbers of gene expression programs (NMF factors) were determined by identifying local stability values preceding a sudden drop. Monodelphis domestica: K = 26; Tenrec ecaudatus: K = 28; Cavia porcellus: K = 31; Mus musculus: K = 48. Homo sapiens: K = 29. Macaca fascicularis: K = 39. g-l. Confusion matrices showing the mean activity score (ranging from 0 to 1) of cells in each cluster, taken from all cells with scores greater than 0.25. m-p. Representative “Type II” and “Type III” decidual gene expression program activity scores across individual stromal cells of human (m), macaque (n), and guinea pig (o) show a diversity from exclusively Type II or Type III to a mixture of both programs. An equivalent comparison of Type I and II in the tenrec is depicted in (p). Silhouettes from PhyloPic under a Creative Commons licence: human, NASA (PDM 1.0); tenrec, Yan Wong (CC0 1.0); macaque, Jane Whitehouse (CC0 1.0); mouse, Michael Keesey (PDM 1.0).
