Extended Data Fig. 6: Role of adhesive ligands in early-stage tumbling-enhanced chondrogenesis.
From: Cell tumbling enhances stem cell differentiation in hydrogels via nuclear mechanotransduction
(a–c) Gene expression of chondrogenic markers after 3 days of chondrogenic induction with adhesive ligand perturbations. N = 3 gels per condition. (a) SOX9 (b) ACAN (c) COL2A1 (d) (Top) Treatment regimen of different adhesive ligand perturbations for the first 3 days in the 21-day chondrogenic induction period. (Bottom) Representative Safranin-O histology on cryo-sectioned samples for sGAG deposition after 21 days of chondrogenic induction with different adhesive ligand perturbations as observed from 3 independent experiments. Scale- 200 μm (shared between all images). ns, not significant; *p < 0.05, **p < 0.005, ***p < 0.001, ****p < 0.0001. P value, one–way ANOVA with Tukey’s multiple comparisons test. Data are presented as mean ± SD for Extended Data Fig. 6a–c.
