Extended Data Fig. 1: Quantification of cell tumbling and nuclear movements upon cytoskeleton inhibition and LINC complex inhibition.
From: Cell tumbling enhances stem cell differentiation in hydrogels via nuclear mechanotransduction
(a) (Left) Centroid speed and (Right) Major axis angular speed upon cytoskeletal inhibition. N ≥ 15 across 3 gels per condition (b) Nuclear movement speed upon cytoskeleton inhibition. N ≥ 20 across 3 gels per condition. (c) (Left) Centroid speed and (Right) Major axis angular speed upon LINC complex inhibition. N ≥ 15 across 3 gels per condition. (d) Nuclear movement speed (5-minute intervals for a total period of 16 hours) upon LINC complex inhibition. N ≥ 20 across 3 gels per condition. ns, not significant; *p < 0.05, **p < 0.005, ***p < 0.001, ****p < 0.0001. P value, one–way ANOVA with Tukey’s multiple comparisons test.
