Fig. 4: Cellulolytic activities of CelABC.
From: A bacterial pioneer produces cellulase complexes that persist through community succession
a, Saccharification of phosphoric acid swollen cellulose (PASC) and Avicel for the culture supernatant, affinity digested preparation (AD) and recombinant CelABC individually expressed in E. coli (see Methods for details). Enzymatic assays are reported as the mean of technical replicates (n = 3) and error bars represent standard error of the mean. b, CMCase-specific activities of the supernatant, AD fraction and in E. coli-expressed CelABC. Enzymatic assays are reported as the mean of technical replicates (n = 3) and error bars represent standard error of the mean. c, SDS–PAGE (8–16% gradient; stained with Coomassie Brilliant Blue) of the AD fraction and E. coli-expressed CelABC. The gel depicted is representative of three gels that displayed very similar results. The gel images were cropped for clarity; the original gel images are provided in Supplementary Fig. 15.
