Extended Data Fig. 5: Histopathological analysis of the SARS-CoV-2 infected mice.
From: Fluorogenic reporter enables identification of compounds that inhibit SARS-CoV-2
H & E analysis of lung sections from the infected mice. K18-hACE2 mice were infected with SARS-CoV-2 (1000 PFU) intranasally with the natural compounds bruceine A, gamabufotalin or vehicle control (solvent) at day 0. Then the inhibitors were delivered intranasally once per day at day 1 and day 2. At day 3 (that is, 3 days post-infection), the lungs were harvested and fixed in 4% PFA and embedding into paraffin and cut at 5 μm slides, stained with hematoxylin and eosin (H & E). Black boxes point to regions of the lung anatomy where inflammation was assessed. The magnified images corresponding to the boxed areas are shown on the right panels. Regions where inflammation was detected are indicated by arrows. Scale bar: 200 μm. The H & E staining was performed in two mice per group and 3 slides per mouse were analyzed.
