Extended Data Fig. 10: NbTPS6 acts as a susceptibility factor against P. parasitica infection.

a, Relative transcript level of GmTPS6 in GmTPS6-overexpressing HC6 hairy roots. n = 3 biologically independent samples for each treatment. b, Overexpression of GmTPS6 in N. benthamiana promotes P. parasitica infection. Leaves transiently expressing YFP or GmTPS6 were inoculated P. parasitica. The pictures were taken at 48 hpi under ultraviolet lamp. c, Lesion length was measured and subjected to statistical analysis. d, Relative biomass of P. parasitica in YFP or GmTPS6-expressed leaves was determined by qPCR. n = 10 (b) and n = 3 (c) biologically independent leaves for each treatment. e, Expression of YFP and YFP-GmTPS6 was confirmed by western blotting using an anti-GFP antibody. Coomassie brilliant blue (CBB) staining as a loading control. The experiment was repeated twice with similar results. f, Phenotype of NbTPS6-silenced N. benthamiana plants using virus-induced gene silencing (VIGS) technique. Four DNA fragments of NbTPS6, including TRV2:VIGS1, TRV2:VIGS2, TRV2:VIGS3 and TRV2:VIGS4 were designed to silence NbTPS6, and the plants inoculated with TRV2:GFP as control. The pictures were taken at 14 dpi. g, Mild chlorotic mosaic symptoms were observed in N. benthamiana leaves inoculated the TRV2:GFP, TRV2:VIGS1, TRV2:VIGS2, TRV2:VIGS3 or TRV2:VIGS4 together with TRV1:00, respectively. V1, V2, V3, V4 represent VIGS1, VIGS2, VIGS3 and VIGS4 fragments of NbTPS6. The pictures were taken at 14 dpi. h, Relative transcript levels of NbTPS6 in four different combinations-silenced leaves. RNA samples were isolated from leaves co-infiltrated TRV1 together with TRV2:GFP, TRV2:VIGS1, TRV2:VIGS2, TRV2:VIGS3 and TRV2:VIGS4, respectively. NbActin gene was used as internal control. i, Disease symptoms of NbTPS6-silenced N. benthamiana leaves and challenged with P. parasitica. j, Lesion length was measured and subjected to statistical analysis. k, Relative biomass of P. parasitica was determined by qPCR. Scale bars: 1.0 cm. n = 3 (h, k) and n = 10 (j) biologically independent leaves for each treatment. Different letters indicate significantly different groups in a, c and d (P < 0.01, unpaired Student’s t test, two-tailed), and in h, i and j (P < 0.01, one-way ANOVA, Tukey HSD). Data of Relative biomass in a, d, h and k represent mean ± s.e.m. In c and j, boxplot centre lines show median value; upper and lower bounds show the 25th and 75th quantile, respectively; upper and lower whiskers show the largest and smallest values, respectively. The experiment was repeated three times with similar results.