Extended Data Fig. 1: Fluorescence in situ hybridization (FISH) micrographs of the highly purified culture (a-f) and enrichment culture (g-o) of ‘Ca. Methylomirabilis sinica’.
From: Methane-dependent complete denitrification by a single Methylomirabilis bacterium
Cells of ‘M. sinica’ were targeted with probe S-*-DBACT-0193−a-A-18 (cy3, red) and bacterial cells were hybridized by EUB I-III (FITC, blue). ‘M. sinica’ appeared in magenta for the double hybridization. a-f, The culture purity was also confirmed based on failure to detect archaeal cells with ARCH915 (cy5, green). g-o, The biomass for FISH was collected from the GLSBR on day 380. m-o, The probe S-*-DARCH-0872−a-A-18 (cy5, green) was used to confirm whether ANME-2d archaea appeared in enrichment cultures. And no obvious signal was detected. The FISH experiments were performed three times with similar results. Scale bars, 5μm.
