Extended Data Fig. 1: Synthetic P2-CaL and P3-P4 fusion peptides are non-toxic.
From: Secretion of the fungal toxin candidalysin is dependent on conserved precursor peptide sequences
a, Damage of oral epithelial cells upon treatment with 40 µM synthetic CaL, synthetic fusion peptides P2-CaL and P3-P4, or a combination of CaL and the respective single peptide for 24 h. Epithelial damage was assessed by quantification of extracellular LDH. Dotted line represents CaL-induced host cell damage (100 %). Statistical analysis was performed with one-way ANOVA and Dunnett’s multiple comparisons test based on the absorbance data, relative to the CaL-treated sample, ***p = 0.001, ****p < 0.0001, n = 4 (biological replicates), more statistical parameters are listed in the Source Data Extended Data Fig. 1, mean ± s.d. b, Scheme of the fusion peptides with respective peptide sequence. The fusion peptide P2-CaL ends with K instead of KR, mimicking Kex1 processing of the mature peptide. In fusion peptide P3-P4, the P3 sequence ends with KR to keep the internal Kex2 processing site, whereas P4 ends with K to mimic the Kex1-processed mature peptide.
