Fig. 3: Divergent thymidine metabolism can be exploited using thymine supplementation to specifically target P. aeruginosa with fluorofolin. | Nature Microbiology

Fig. 3: Divergent thymidine metabolism can be exploited using thymine supplementation to specifically target P. aeruginosa with fluorofolin.

From: A folate inhibitor exploits metabolic differences in Pseudomonas aeruginosa for narrow-spectrum targeting

Fig. 3

a, Growth of E. coli MG1655 or b, P. aeruginosa PA14 in 0.3 mM TMI-supplemented media and treated with fluorofolin at 2× MIC or DMSO. Curves represent average OD600 of 2 biological replicates. c, Competition of P. aeruginosa PA14 and E. coli MG1655 in LB or TMI-supplemented LB media. PA14 and E. coli MG1655 were inoculated at a 1:1 ratio and grown overnight in the presence or absence of 50 μg ml−1 fluorofolin in each media condition. The following day, c.f.u.s were counted on LB agar or Pseudomonas selection agar plates to determine c.f.u. ml−1 of each species. Data represent mean ± s.d. of 3 biological and 3 technical replicates. ***P ≤ 0.001, P = 0.0006; calculated using two-sided unpaired t-test using Prism 9. d, Representative culture images.

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