Fig. 5: Comprehensive transcriptome-wide gene fitness profiling of phage T4 using pooled CRISPRi-ART.
From: CRISPRi-ART enables functional genomics of diverse bacteriophages using RNA-binding dCas13d
a, Experimental library screening pipeline to discover phage genes important for efficient phage infection. b, Volcano plot summarizing phage functional genomics screen for phage T4 (273 coding sequences). Representative Fit and Semi-fit genes are highlighted in red and orange, respectively. Thresholds for significance and fitness in volcano plots are shown with a dashed line. c, Distribution of Fit, Semi-fit and Not-fit genes in phage T4 binned and coloured by essentiality as reported previously25. Antitoxin dmd, probably essential in this RnlAB+ E. coli strain, is labelled. d, Phage T4 transcriptome-wide fitness landscape. The outer track displays the phage gene map with genes categorized by function, and the inner track shows fitness measured for each corresponding gene, coloured by Fit (red), Semi-fit (orange) or Not fit (blue). For b and d, gene fitness is shown as the mean of 3 biological replicates with log10-transformed unidirectional K–S P value. crRNA fitness is shown as the median of 3 biological replicates.
