Extended Data Fig. 9: UL141 promotes endothelial cell tropism independently of the pentamer complex.
From: The GATE glycoprotein complex enhances human cytomegalovirus entry in endothelial cells
a, Representative images of UL141-dependent spread in endothelial cells. Fibroblasts and endothelial cells were infected with 50 or 100 genome equivalents/cell, respectively, of 141- or 141 + TB40/E viruses produced by fibroblasts. Cells were stained for IE1 (green) and Hoechst (blue) at the indicated days post-infection to monitor viral spread. Scale bars denote 800 μm. b, Low MOI (0.01 TCID50) viral growth kinetics in HUVEC infected with 141- or 141+ viruses up to 14 dpi (n = 3). Data were logarithmically transformed to fit a Gaussian distribution prior to calculating statistical significance via 2-way ANOVA (two-tailed). ***P = .0009 for 12 dpi data points. c, Non-reducing SDS-PAGE of HUVEC cell lysates and HUVEC-derived virions. Cells were infected with 141- and 141+ viruses to measure virion incorporation of known HCMV entry complexes, trimer (gH/gL/gO) and pentamer (gH/gL/128). Lysates were immunoblotted for gL to identify covalently-linked entry complexes, major capsid protein (MCP) to measure virion abundance, and UL148 to assess the purity of the virion preparations. d, Quantification of band intensities for gH/gL/gO and gH/gL/128 abundance in HUVEC-derived virions from two independent experiments. Band intensities of 141- and 141+ virions were normalized to MCP. Error bars represent ± SEM.
