Fig. 5: Quartet immunization induces broad antibodies even after a preprimed SARS2 response.
From: Proactive vaccination using multiviral Quartet Nanocages to elicit broad anti-coronavirus responses
a, Summary of timeline and antigens for this set of immunizations. b, ELISA for serum IgG to SARS2 Wuhan RBD presented as the AUC of a serial dilution. All mice were primed with Wuhan SARS2 Spike, before boosting with Wuhan SARS2 Spike protein (light green), Homotypic Nanocage (pink), Mosaic-8 (dark blue), SpyTag-Quartet Nanocage (red), Dual Quartet Nanocage (orange), Quartet Nanocage with SARS1 RBD replacing SARS2 (purple) or Dual Quartet Nanocage with SARS1 RBD replacing SARS2 (cyan). Solid rectangles under samples indicate ELISA against a component of that vaccine (matched). Striped rectangles indicate ELISA against an antigen absent in that vaccine (mismatched). Each dot represents one animal. The mean is denoted by a bar ±1 s.d.; n = 6. c, ELISA for serum IgG to other sarbecovirus RBDs, as for b, with each dot representing one animal and the mean being denoted by a bar ±1 s.d.; n = 6. Significance was calculated with an ANOVA test using Tukey’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001; other comparisons were non-significant.
