Extended Data Fig. 8: The flexibility of our SACD concept on other fluctuation-based SR techniques.
From: Enhanced detection of fluorescence fluctuations for high-throughput super-resolution imaging
(a) A representative live COS-7 cell labelled with Skylan-S-TOM20 imaged by SD-confocal (bottom) and SACD using 20 frames (top) (Supplementary Video 3). (b) Comparison of results (enlarged regions enclosed by the white box in a) of SD-confocal (1st row, averaged by 20 frames), SOFI (2nd row, reconstructed by 20 frames), HAWK-SOFI (3rd row, reconstructed by 20 frames), and SACD (4th row, reconstructed by 20 frames). (c) Comparison results (enlarged regions enclosed by the white box in a) of SRRF with temporal radiality average (SRRF-TRC, top left), SACD assisted SRRF-TRC (bottom left), SRRF with temporal radiality autocorrelation (SRRF-TRAC, top middle), SACD assisted SRRF-TRAC (bottom middle), ESI with second-order (ESI-2nd, top right), and SACD assisted ESI-2nd (bottom right). (d) Highlighted regions from the white box in (b). Details in Supplementary Note 6. Experiments were repeated ten times independently with similar results. Scale bars: (a) 5 μm; (b, c) 3 μm; (d) 500 nm.
