Extended Data Fig. 1: CD27 expression on T_H17 cells during autoimmunity, and cellular homeostasis of CD27⁺ and CD27⁻ T_H17 subsets.

a, Analysis of publicly available single-cell transcriptomics data from IL-17–GFP⁺ cells in dLN compared with the CNS⁸. b, Flow cytometry analysis of putative memory or activation markers on CD4⁺ TCR-β⁺ YFP⁺ cells from the dLN and spleen of mice at day nine post MOG-immunization. c, Summary of CD27 expression on CD4⁺ TCR-β⁺ YFP⁺ cells in dLN (red; n = 5, day 5; n = 3, day 7; n = 7, day 9; n = 8, day 16) overlaid with clinical EAE score (black, n = 5). d, Ki-67 expression in CD27⁺ and CD27⁻ cell populations. e, Flow cytometry analysis (left) and summary (right) of CD27 expression on transferred CD27⁺ or CD27⁻ YFP⁺ cells, at day 14 after transfer into CD45.1⁺ hosts (n = 14, CD27⁺; n = 15, CD27⁻). f, Flow cytometry analysis of Bcl-2 expression in CD27⁺ and CD27⁻ populations. Data are means ± s.e.m. and representative of three (b–d, f) or at least five (e) independent experiments. Numbers in plots represent frequencies of cells in gates; numbers within histograms represent mean fluorescence intensities. The Mann–Whitney U-test (two-sided; non-parametric) was used in e to determine statistical significance.

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