Extended Data Fig. 1: Overlap of H3K36me3 with m6A and co-expression of their writer genes.
From: Histone H3 trimethylation at lysine 36 guides m6A RNA modification co-transcriptionally
a, Overlaps of m6A sites with histone modification sites in the human genome. Histogram showed the observed and expected percentages of m6A sites that overlap with various histone modification sites; the ENCODE ChIP–seq data and m6A-seq data (Gene Expression Omnibus (GEO) accession GSE37003) obtained from HepG2 cells were used for the analyses. b, Distance of histone modifications to the nearest m6A peaks. Input and H3K27me3 were shown as negative controls. c–e, Correlation of SETD2 with individual m6A MTC genes (that is, METTL3, METTL14 and WTAP) in expression in normal tissues (c), cancer samples (d), and cell lines (e), based on the data from Genotype Tissue Expression (GTEx), The Cancer Genome Atlas (TCGA), and Cancer Cell Line Encyclopedia (CCLE) databases, respectively. Note that every dot represents one tissue type (c) or one cancer type (d). f, Co-expression analysis of SETD2 and individual m6A MTC genes in 33 cancer cell lines based on our qRT-PCR data. The value of each gene represents relative expression as normalized to HEK293T cells. Correlation coefficient (r) and P values were calculated by Pearson’s correlation analysis.
