Extended Data Fig. 5: Locus-specific regulation of m6A by H3K36me3.
From: Histone H3 trimethylation at lysine 36 guides m6A RNA modification co-transcriptionally
a, b, Schematic showing the epigenetic editing of H3K36me3 by dCas9–KDM4A on the MYC CRD (a) or by dCas–SETD2 on GNG4 (b). c, Distribution of H3K36me3 and m6A peaks across the GNG4 mRNA transcript. d, Schematic showing endogenous GNG4 and artificial MYC-GNG4 fusion gene. The primers used to distinguish endogenous GNG4 (GNG4 5′UTR), MYC-GNG4 fusion or GNG4-vector fusion (GNG4 pcDNA) sites were indicated by black, orange or blue arrows, respectively. e, Quantification of H3K36me3 (top) and m6A (bottom) in endogenous GNG4 or MYC-GNG4 fusion genes by ChIP–qPCR or gene specific m6A assay in control (shCtrl) or SETD2-knockdown (shSETD2#1) HEK293T cells. Data are mean ± s.d. of five independent experiments. ***P < 0.001; two-tailed Student’s t-test.
