Extended Data Fig. 8: Editing with SpCas9 and LbCas12a at endogenous microduplications.
From: Precise therapeutic gene correction by a simple nuclease-induced double-stranded break
a, Percentage of microhomology-mediated deletions out of total indels at endogenous sites in cells treated with SpCas9 and LbCas12a. Mean ± s.e.m., dots represent individual data points from three biological replicates. b, Schematic of endogenous site containing a 24-bp microduplication for SpCas9 target sites 1–3. The 24-bp microduplication repeats are shown in bold red and blue. The PAM sequence is outlined in magenta and the protospacer sequence is underlined. Magenta carets indicate the site of DSB. c, Percentage of alleles with 24-bp deletion (green) and total indels (blue) for all three guides from TIDE analysis. Guide 3 produces primarily 23-bp deletions, but not 24-bp deletions, probably because it recuts the collapsed DNA sequence. Bars shows the mean from n = 3 biological repeats, individual data points are represented by dots. d, Proportion of the 24-bp deletion out of total indels as individual data points (dots), with mean ± s.e.m. n = 3 biological repeats. e, Schematic of endogenous site containing a 27-bp microduplication for SpCas9 target sites 1 and 2. f, Percentage of alleles with 27-bp deletion (green) and total indels (blue) for both guides from UMI-based Illumina deep sequencing. Bars show the mean from n = 3 biological repeats, individual data points are represented by dots. g, Proportion of the 27-bp deletion out of total indels as individual data points (dots) with mean ± s.e.m. n = 3 biological replicates.
