Extended Data Fig. 3: PacBio long-read sequencing analysis of SpCas9-edited LGMD2G iPS cells clones and a complex colony at the TCAP locus.
From: Precise therapeutic gene correction by a simple nuclease-induced double-stranded break
IGV graphs depicting representative reads obtained for clonal isolates of edited LGMD2G iPS cells (Fig. 1d), spanning a genomic region of about 2,035 bp surrounding the TCAP target site. The genotype of the clones (deduced by Illumina deep sequencing) is indicated beside an enlargement of the TCAP target region within the PacBio data. The sequences of the two alleles (listed above the IGV plot) obtained from sequencing are shown with repeats in red and blue. Alleles that reverted to wild-type as a result of collapse of microduplication are half red/half blue. Bottom, IGV plot for one complex iPS cell colony that appears to have been nucleated by more than one cell, with large deletions present in the genome (sizes indicated).
