Extended Data Fig. 9: Suppressed HIV-1JR-FL that carries amber positions in gp120 and gp41 enables smFRET imaging of Env from two distinct perspectives.
From: Associating HIV-1 envelope glycoprotein structures with states on the virus observed by smFRET
a, Schematic of tagged sites in HIV-1JR-FL Env that were used for enzymatic labelling and amber stop codon (TAG)-suppressed incorporation of unnatural amino acids for click labelling. HIV-1JR-FL V1–Q3 V4–A1 carries the Q3 peptide in the V1 loop and the A1 peptide in the V4 loop. HIV-1 JR-FL V1–Asn136TAG V4–A1 carries a TAG at position Asn136 in V1 and the A1 peptide in V4. HIV-1JR-FL V4–A1 α6–Arg542TAG carries the A1 tag in gp120 V4 and a TAG at Arg542 in the α6 helix of gp41. b, c, Neutralization of HIV-1JR-FL wild type, 100%-peptide-tagged V1–Q3 V4–A1, 100%-amber-suppressed V1–Asn136TAG V4–A1 and V4–A1 α6–Arg542TAG by sCD4D1D2–Igαtp (b), and eCD4-Ig(Q40A, mim2) (c). Neutralization curves (b, c) represent mean ± s.d. from three replicates in triplicates.
