Extended Data Fig. 8: Sorting purity of mouse ILCs.
From: Group 3 innate lymphoid cells mediate early protective immunity against tuberculosis
a, C57BL/6 mice were aerosol-infected with approximately 100 CFU Mtb and lungs were collected at 5 d.p.i. The lung CD45 population was enriched by using CD45 microbeads. CD45-enriched cells were stained and lung ILCs (CD45+CD127+Lin−NK1.1−) were purified by using FACSJazz. Sort purity is shown here. b, mRNA expression of Ccr6, Rorγt and Ahr relative to Gapdh in the purified ILCs (CD45+CD127+Lin−NK1.1−) and non-ILC population (CD45+CD127−Lin−NK1.1−) were quantified by RT–PCR. Significance is calculated by two-way ANOVA, ****P < 0.0001. Data are mean ± s.d. and individual values.
